New PDF release: Candida albicans: Methods and Protocols

By T. Sreevalsan (auth.), Ronald L. Cihlar, Richard A. Calderone (eds.)

ISBN-10: 1588297608

ISBN-13: 9781588297600

ISBN-10: 1603271511

ISBN-13: 9781603271516

Over the process the earlier decade, there were impressive advances within the examine of human pathogenic fungi. those advancements have taken position all through a variety of disciplines, and feature come because the results of newly to be had genome sequences of pathogens corresponding to candida albicans and different version fungi. In Candida Albicans: tools and Protocols, professional researchers discover those interesting new insights, targeting the research of medically very important fungi and Candida spp particularly. Chapters research severe facets of molecular tools, offering details on reporter gene assays, transformation, gene expression in vivo, and techniques for large-scale gene disruption. even as, the paintings contains in-depth descriptions of ailment types of candidiaisis, evidence approximately pressure id, and instructions at the training of samples for proteomic investigations and tandem affinity purification. Composed within the hugely winning Methods in Molecular Biology™ sequence layout, every one bankruptcy includes a short creation, step by step equipment, an inventory of worthwhile fabrics, and a Notes part which stocks pointers on troubleshooting and warding off identified pitfalls.

Authoritative and leading edge, Candida Albicans: tools and Protocols is a useful resource of equipment for investigators within the exhilarating fields of scientific and molecular mycology.

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Is somewhat problematic. 2-mL volume of any antigen (or cells) is used for immunization or infection. A restrainer is required in which the mouse is placed such that the tail protrudes through one end of the restrainer. Also required is a source of light that is placed beneath the tail vein to ensure its localization. Following injection, the injection site is cleansed and animals are returned to their cages. Animals should be monitored at least two to three times per day and moribund mice euthanized.

1999). Rat model of Candida vaginal infection. A. ), Academic Press, New York, pp. 735–740. 21. R. and Fidel, J. R. (2002). Partial protection against experimental candidiasis after mucosal vaccination with heat killed Candida albicans and mucosal adjuvant LT (R192G). Med. Mycol. 40, 291–299. 22. , Girolamo, A. and Cassone, A. (2002). Intravaginal and intranasal immunizations are equally effective in inducing vaginal antibodies and conferring protection against vaginal candidiasis. Infect. Immun.

Naı¨ve or compromised), and all may have an impact on the vaccination procedure and outcome. c. injections of the antigen. , whole cell, partially or purified antigen, and the use (or not) of an adjuvant, described above. p. c. p. has been employed. Some studies involved infection by the oral route – to partially imitate the natural pathogenesis of candidiasis, originating from an endogenous source – the gastrointestinal (GI) tract. Specifically, the protocol described below uses the Candida albicans r-Als (recombinant Als) antigen.

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Candida albicans: Methods and Protocols by T. Sreevalsan (auth.), Ronald L. Cihlar, Richard A. Calderone (eds.)

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