Get Biomembranes Part G: Bioenergetics: Biogenesis of PDF

By Sidney Fleischer, Lester Packer (Eds.)

ISBN-10: 0121819566

ISBN-13: 9780121819569

The significantly acclaimed laboratory normal, Methods in Enzymology, is likely one of the such a lot hugely revered guides within the box of biochemistry. due to the fact 1955, every one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this day - actually a necessary book for researchers in all fields of existence sciences

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Extra info for Biomembranes Part G: Bioenergetics: Biogenesis of Mitochondria, Organization, and Transport

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36 These trial precipitations are conveniently carried out for 8-16 hr at 4 ° in small plastic Eppendorf centrifuge tubes (capacity - 1 . 5 ml) which can be used with a microcentrifuge man3z G. Schatz and J. Saltzgaber, Biochem. Biophys. Res. Commun. 37, 996 (1969). 3a F. Cabral and G. Schatz, this volume [54]. 34 A. Tzagoloff and P. Meagher, J. Biol. Chem. 246, 7328 (1971). 35 T. L. M a s o n and G. Schatz, J. Biol. Chem. 248, 1355 (1973). 36 E. Ebner, T. L. Mason, and G. Schatz, J. Biol. Chem.

Work, Biochem. J. 80, 9 (1961). 2o D. S. Beattie, R. E. Basford, and S. B. Kortiz, Biochemistry 6, 3099 (1967). 21 M. N. Gadaleta, M. Greco, G. Del Prete, and C. Saccone, Arch. Biochem. Biophys. 172, 238 (1976). 22 N. G. Ibrahim, J. P. Burke, and D. S. Beattie, J. Biol. Chem. 249, 6806 (1974). 22 BIOGENESIS OF MITOCHONDRIA [2] respiratory-chain-linked phosphorylations. TM Equal rates of amino acid incorporation are obtained in our laboratory using either energy source; however, other groups ~,4 have reported a preference for either one of the systems.

Amsterdam, 1976. 23 G. Schatz and J. Saltzgaber, Biochem. Biophys. Res. Commun. 37, 996 (1969). 34 BIOGENESIS OF MITOCHONDRIA [3] N e u r o s p o r a crassa by Sebald et al. 24 It has later been used in other sys- tems, including mammalian cells in tissue culture. References to the literature are given in Table I. Different aspects and limitations of the method have been discussed previously. 1,5,19 One point to stress here is the toxicity of drugs especially such as cycloheximide. Long-term experiments with monolayer cultures of mammalian cells, for instance, are not possible because the cells detach from the disks unless extremely low doses are used.

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Biomembranes Part G: Bioenergetics: Biogenesis of Mitochondria, Organization, and Transport by Sidney Fleischer, Lester Packer (Eds.)


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