By K. K. Conzelmann (auth.), Professor Yoshihiro Kawaoka DVM, PhD (eds.)
Negative-strand RNA viruses, so named as a result of the polarity in their genomic RNA to mRNA, contain vital human and non-human pathogens. opposite genetics, a method that permits iteration of recombinant viruses, was once first constructed for negative-strand RNA viruses in 1989. considering then, it has speeded up the velocity of study on those viruses, resulting in a wealth of recent wisdom that in a different way may were tough to acquire. remarkable achievements that may be attributed to opposite genetics contain the creation of inactivated and reside vaccine applicants and of vaccine and gene move vectors.
This quantity covers significant advances in opposite genetics suggestions during the last decade, state of the art uncomplicated technology and the scientific implications of experimental findings. this could re-ignite curiosity in negative-strand RNA viruses between readers, together with these in different disciplines, resulting in extra growth in knowing those very important viruses and in constructing potent measures of regulate.
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Additional info for Biology of Negative Strand RNA Viruses: The Power of Reverse Genetics
Acknowledgements. Research in the author's lab is supported by grants from the Deutsche Forschungsgemeinschaft (SFB 455-A3, and SPP1089 C0260/1-1) and the European Commission (QLK2-CT-1999-00443 and QLK2-CT-2002-81399). Critical reading of the manuscript by Stefan Finke and Birgit Bossert is greatly appreciated. References Aoki Y, Aizaki H, Shimoike T, Tani H, Ishii K, Saito I, Matsuura Y, Miyamura T (1998) A human liver cell line exhibits efficient translation of HCV RNAs produced by a recombinant adenovirus expressing T7 RNA polymerase.
JGen Virol 77 (Pt 5):963-967 Buchholz UJ, Finke S, Conzelmann KK (1999) Generation of bovine respiratory syncytial virus (BRSV) from eDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. J Virol 73:251-259 Buchholz UJ, Granzow H, Schuldt K, Whitehead SS, Murphy BR, Collins PL (2000) Chimeric bovine respiratory syncytial virus with glycoprotein gene substitutions from human respiratory syncytial virus (HRSV): effects on host range and evaluation as a live-attenuated HRSV vaccine.
The latter can be further separated into polymerase proteins and vRNA by cesium trifluoroacetate gradient centrifugation (Honda et al. 1988, 1990). Parvin et al. (1989) first reconstituted synthetic viral RNA into functional vRNP complexes. Plasmid DNA encoding short model influenza viral RNA encompassing the genomic termini was transcribed in vitro from the T7 RNA polymerase promoter and the resulting RNA transcript was then mixed with purified polymerase and NP proteins, leading to functional vRNP complexes.
Biology of Negative Strand RNA Viruses: The Power of Reverse Genetics by K. K. Conzelmann (auth.), Professor Yoshihiro Kawaoka DVM, PhD (eds.)