K. Mühlethaler (auth.), Professor Dr. Giorgio Semenza,'s Biochemistry of Membrane Transport: FEBS — Symposium No. 42 PDF

By K. Mühlethaler (auth.), Professor Dr. Giorgio Semenza, Professor Dr. Ernesto Carafoli (eds.)

ISBN-10: 3642665640

ISBN-13: 9783642665646

ISBN-10: 3642665667

ISBN-13: 9783642665660

This quantity comprises the lawsuits of the FEBS Sym­ posium at the Biochemistry of Membrane delivery, which used to be held on the Swiss Institute of know-how, Zlirich, July 18-23, 1976. Of the audio system invited or­ iginally, simply 5 couldn't attend the assembly, and of the lectures given, all yet one of many texts are released the following. hence, this quantity offers a devoted ac­ count number of how the assembly used to be initially conceived and really came about. This Symposium on Biochemistry of Membrane delivery was once the 1st Symposium backed by means of the FEBS outdoors the once a year FEBS-Meetings, after the specific assembly on commercial Biochemistry, which happened in Dublin in 1973, and it displays the curiosity and the fashion for gatherings of smaller measurement than the reliable FEBS conferences. the subject of the Symposium was once a simple selection, not just simply because membrane delivery is becom­ ing a growing number of very important to biochemistry each year, but additionally a result of long-standing curiosity of Swiss technology within the box. within the number of the themes and of the audio system, efforts have been made to accomplish as balanced a assurance of the realm as attainable. even if, in view that a few features of membrane biochemistry have been handled widely on the parallel tenth Inter­ nationwide Congress of Biochemistry in Hamburg, GFR, cer­ tain subject matters got much less emphasis than others. The Symposium was once attended through approximately four hundred members (we anticipated 200-250); between them forty eight have been invited audio system, and a few two hundred contributed posters.

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Mol- 1 above and below the transition temperature, respectively. Cholesterol has been shown to decrease the rate of permeation of non-electrolytes across lipid bilayers in the liquid-crystalline state. It is well-known that cholesterol reduces the energy content of the lipid phase transition and it was found of interest to investigate the temperature dependency of water permeation across liposomes of mixtures of synthetic phospholipid with cholesterol. , 1969). The effect of cholesterol on water permeation in liposomes of dipalmitoyl lecithin is demonstrated in Figure 2.

The temperature dependency of the osmotic shrinkage of these liposomes shows that above the transition temperature the rate of permeation of water is decreased by the presence of cholesterol. Increasing amounts of cholesterol cause a gradual disappearance of the abrupt change in the rate of water permeation near the gel to liquid-crystalline phase transition of liposomes of synthetic saturated lecithins. At cholesterol concentrations above 30 mol% no further discontinuity in the rate of water permeation exists.

However, crosslinking of the aggregated integral proteins at -13°C with 1% glutaraldehyde prevented free lateral translational diffusion of integral proteins when the membrane was returned to above the transition temperature (Fig. 14). Thus the low temperature-induced separations between integral proteins and gel-state lipid were maintained after the gel-state lipid was permitted to melt (Fig. 14). Under these conditions two types of smooth lipid regions were noted above the transition temperature.

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Biochemistry of Membrane Transport: FEBS — Symposium No. 42 by K. Mühlethaler (auth.), Professor Dr. Giorgio Semenza, Professor Dr. Ernesto Carafoli (eds.)


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