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Изображения: черно-белые рисункиHiermit genehmige ich die D. (Luft) T. 2109 G-#246;/U four Teil zero — N. f. D. „Bf 109 G-6/U4 Flugzeug-Handbuch Teil zero: Allgemeine Angaben (Stand Dezember 1943) Ausgabe Februar 1944. Sie tritt mit dem Tage der Herausgabe in Kraft.

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1. Prepare a solution of 2-naphtaldehyde 4 (10 g, 64 mmol) in acetic anhydride (25 mL). 2. 75 g, 83 mmol). 3. 4 mmol). 28 P. D’ Arrigo and D. Tessaro Fig. 3. Synthesis of 2-naphthyl pyruvic acid (2-NpPA) 6. 4. Heat the mixture to 100°C in an oil bath for 2 h. 5. Stir the reaction mixture at room temperature overnight to allow the corresponding azalactone 5 to precipitate as an yellow solid. 6. Add 25 mL of water. 7. Filter the solid, wash three times with acetone and dry under reduced pression to give product 5 (8 g, 52% yield) of suitable purity.

Caligiuri A, D’Arrigo P, Gefflaut T et al. (2006) Multistep enzyme catalysed deracemisation of 2-naphthyl alanine. Biocatal Biotransfor 24, 409–413. 17. Fantinato S, Pollegioni L, and Pilone M S (2001) Engineering, expression and purification of a His-tagged chimeric D-amino acid oxidase from Rhodotorula gracilis. Enzyme Microb Tech 29, 407–412. 18. Kagamiyama H and Hayashi H (2000) Branchedchain amino-acid aminotransferase of Escherichia coli. Method Enzymol 324, 103–113. 19. Kamitori S, Hirotsu K, Higuchi T et al.

2. Keep the resulting mixture at 60°C under stirring for 30 min, and then bring to dryness using a rotary evaporator. 3. Resuspend the resulting white solid in 5 mL of diethyl ether and filter through a Buchner funnel, obtaining the final product L-2-NpAla whose enantiomeric excess is measured by HPLC. 4. Notes 1. The 1H NMR spectrum of D,L-2-NpAla has been recorded in DMSO-d6/TFA-d4 because of the low solubility of the compound. 2. The large amount of pyruvic acid produced clearly indicates that the use of cysteine sulphinic acid as amino donor was very effective in completely shifting the equilibrium.

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Bf-109 G-6-U4 Flugzeug-Handbuch by Язык: немецкий

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