New PDF release: Analytical Biochemistry

By David Holme, Hazel Peck

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L. (1991). Adsorption of a-helical antifreeze peptides on specific ice crystal planes. Biophysical Journal, 56, 409-18. L. (1993). Adsorption of fish antifreeze glycopeptides to ice, and effects on ice crystal growth. Biophysical Journal, 64, 252-9. A. L. (1994). Effects of a polymeric, nonequilibrium "antifreeze" upon ice growth from water. Journal of Crystal Growth, 143, 301-10. L. E. (1970). Studies of the structure of freezing point-depressing glycoproteins from an Antarctic Fish. Journal of Biological Chemistry, 245, 2909-13.

It is bound to the endoplasmic reticulum in animal and yeast cells (Enoch, Catala & Strittmatter, 1976; Strittmatter et ai, 1974) where it forms the terminal component of a multicomponent system which includes cytochrome b5 and the NADHdependent cytochrome b5 reductase. This complex catalyses the insertion of a double bond at the 9-10 position in a variety of saturated fatty acid-CoA substrates (mainly stearoyl- and palmitoyl-CoA) and accounts for all de novo synthesized ds-unsaturated fatty acids.

Early experiments indicated that the homology at the nucleotide level between carp and rat was too low to allow detection of a desaturase mRNA by stringent Northern analysis. However, subsequent work on isolated carp hepatocytes indicated that continued long-term culture led to a large isothermal desaturase induction (Macartney, Tiku & 0 Days 6225 nt 3911 nt 2800 nt 872 nt In vivo cold induced Hepatocyte culture at 30 °C Probe: Carp liver A9-desaturase clone, pcDsLJ Fig. 1. Northern analysis of hepatic RNA samples using a probe derived from the carp liver desaturase clone, pcDsL7.

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Analytical Biochemistry by David Holme, Hazel Peck


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