By Emma Holečková, Vincent J. Cristofalo (auth.), Emma Holečková, Vincent J. Cristofalo (eds.)
The annual assembly of the eu Tissue tradition ., Society used to be held on the fortress of Zinkovy in Czechoslovakia from may well 7-10,1969. integrated as a part of this assembly was once a symposium on "Aging in cellphone and Tissue Culture." This quantity includes the papers awarded at that symposium. using mobilephone and tissue tradition suggestions to check the mechanism of getting older isn't new. for instance, it has lengthy been recognized that age-associated alterations which happen in plasma can inhibit phone proliferation in vitro; additionally that the time lapse sooner than mobile migration from ex planted tissue fragments raises with expanding age. those are either examples of the expression in vitro of getting older in vivo. extra lately, realization has been excited about the prevalence of senescence in vitro. those investi gations have integrated stories of adjustments in non dividing mobile cultures, and to a a bit better quantity, of age-related alterations within the proliferative means of cells in vitro. for instance, cells derived from human fetal lung keep many houses of standard cells together with a strong common diploid karyotype and those cultures were proven to have a constrained life-span in vitro. In addi tion, cultures derived from human grownup lung convey a similar common features and seem to have a shorter lifestyles span than cells derived from fetal lung.
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Additional resources for Aging in Cell and Tissue Culture: Proceedings of a symposium on “Aging in Cell and Tissue Culture” held at the annual meeting of the European Tissue Culture Society at the Castle of Žinkovy in Czechoslovakia, May 7–10, 1969
This finding is correlated with changes in the size distribution of the cells and suggests the induction of instability into the cells by the environment. One problem arising from these findings is to determine whether the aging in vitro is due to processes of cell selection or to the induction of cellular instability simultaneously in all cells of the cell population. In this paper, aging has been studied using diploid , embryonic heart cells of the marsupial Potorous tridactylis. These cells are characterized by six pairs of chromosomes.
Figure 4 shows the results of hydroxyproline analysis on part of the material which we used for cultivation. To our surprise, a considerable amount of hydroxyproline was present in both the trypsinized cell samples, but after the Hyp/dry weight(pg/100mg) Hyp/N2 (pg/mg) % cz:o 200 200 o young o young • old • old Cells T 100 100 Cells T Cells TC Fig. 4. The amount of hydroxyproline expressed as percentage (the amount of hydroxyproline in the young rat's kidney tissue remaining after trypsinization = 100%).
The optimal growth span was obtained with medium F-15 which consisted of 5% filtered bovine embryo extract, 10 %normal horse serum and 85 %solution V-614. Z .... C) 0 X MEM 5 -5TPB )(/0 30 X/O o 0 ~ C( X X~-~ XX ....... o 0 0 ~MEM 24 10-5EE 18 12 6 20 40 60 80 100 120 140 160 180 DAYS Fig. 6. Growth span of chick muscle fibroblasts in two different media. Strains represented by dark circles and by crosses were established from the same initial cell pool. The strain indicated by open circles was from a separate pool of cells.
Aging in Cell and Tissue Culture: Proceedings of a symposium on “Aging in Cell and Tissue Culture” held at the annual meeting of the European Tissue Culture Society at the Castle of Žinkovy in Czechoslovakia, May 7–10, 1969 by Emma Holečková, Vincent J. Cristofalo (auth.), Emma Holečková, Vincent J. Cristofalo (eds.)